【摘要】 建立了PCR快速鉴定啤酒有害菌的新方法。用基于抗酒花基因horA部分序列的特异引物对啤酒污染乳酸菌进行PCR检测,灵敏度可达到3个细胞(CFU),样品的预培养需12~16h。啤酒有害菌检测所需时间从传统的5d减少到24h。更多还原

【Abstract】 A new method for rapid detecting beer-spoilage by PCR was developed.This method is based on the sequence of a hop-resistance gene horA,which a pair of specific primers were designed based on the partial sequence of it.Using the primers,beer-spoilage Lactobacillus species were detected by PCR.The sensitivity was reached to 3CFU.The pre-enrichment of samples was needed only 12~16 h.The duration for beer-spoilage determination by PCR(24h)was markly shorter than by conventional method(5~7d).更多还原