【摘要】 目的研究二氮嗪(DZ)开放线粒体ATP敏感性钾通道对缺血再灌注损伤所致大鼠心肌组织ICAM-1和TNF-α表达的影响。方法采用离体工作心脏灌注模型,大鼠随机分为正常对照组,缺血再灌注组,DZ治疗组,DZ抑制剂-格列苯脲组。采用RT-PCR法测定心肌ICAM-1mRNA表达,ELISA检测试剂盒检测TNF-α蛋白含量。结果与正常对照组相比,心肌组织ICAM-1表达和TNF-α含量在缺血再灌注后有显著性增加(P<0.01)。DZ处理后,ICAM-1表达和TNF-α含量与缺血再灌注组相比有明显下降(P<0.05);格列苯脲组ICAM-1表达和TNF-α含量明显高于DZ治疗组(P<0.05)。结论心肌缺血再灌注损伤使ICAM-1和TNF-α表达明显升高,二氮嗪(DZ)开放线粒体ATP敏感性钾通道可下调心肌组织ICAM-1和TNF-α的表达,这可能有助于减轻缺血再灌注所致心肌损伤。更多还原

【Abstract】 OBJECTIVE To investigate the effect of diazoxide on expression of ICAM-1 and TNF-α during ischemia-reperfusion in rat cardiac muscles.METHODS Using isolated rat heart model,40 male SD rats were randomly divided into normal control group(n=10),ischemia-reperfusion group(n=10),diazoxide treatment group(n=10)and diazoxide inhibitor group(n=10).The expression of ICAM-1mRNA was extracted and measured by RT-PCR.TNFα level in the myocardium were measured using enzyme-linked immunosorbent assay(ELISA) system. RESULTS The expression of ICAM-1mRNA significantly increased after ischemia-reperfusion and TNF-α levels had the same change.treatment with diazoxide significantly inhibited the expression of ICAM-1mRNA,and concomitantly decreased the level of TNF-α in the myocardium.The diazoxide inhibitor can significantly increase the expression of ICAM-1mRNA and the level of TNF-α.CONCLUSION These data suggest that myocardial ischemia-reperfusion can result in intensive activation of ICAM-1 and TNFα.Early treatment with diazoxide might be benefit for attenuation of myocardial injury induced by ischemia-reperfusion.更多还原