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重离子束诱变呼吸缺陷型酵母菌株的筛选与快速鉴定
Screening and Identification of Respiration Deficiency Mutants of Yeasts(Saccharomyces Cerevisiae) Induced by Heavy Iron Irradiation
【摘要】 利用单核能为5.19 MeV/u的22Ne5+辐照啤酒酵母菌株,采用TTC筛选培养基筛选出呼吸缺陷型酵母菌株。通过一种新型而简便的限制性酶切分析手段,有效地对所筛选出的8株呼吸缺陷型酵母菌株进行遗传学鉴定。
【Abstract】 A screen of respiration deficiency mutants of Saccharomyces Cerevisiae induced by 5.19 MeV/u 22Ne5+ ion irradiation is studied.Some respiration deficiency mutants,which are white colony phenotype in the selective culture of TTC medium,are obtained.The mutants are effectively identified by means of a new and simplified restriction analysis method.
【关键词】 重离子束;
诱变;
呼吸缺陷型;
酵母菌株;
【Key words】 heavy ion irradiation; mutant; respiration deficiency; yeast;
【Key words】 heavy ion irradiation; mutant; respiration deficiency; yeast;
- 【文献出处】 同位素 ,Journal of Isotopes , 编辑部邮箱 ,2006年01期
- 【分类号】Q933
- 【被引频次】7
- 【下载频次】206