【摘要】 微生物通过发酵产酶可以将植物单宁降解成小分子酚类化合物或其衍生物,但培养条件对其产酶影响很大。论文采用固态培养法,对黑曲霉产生塔拉单宁水解酶的条件进行了研究。结果表明,当培养液中塔拉单宁浓度为75 g/L、葡萄糖浓度为3 g/L、(NH4)2SO4浓度为0.2 g/L2、50 mL锥形瓶装液量为25 mL、惰性载体用量为5.6%(w/v)、起始pH为5.5、接种量为12%(v/v)、30℃培养72 h时,该黑曲霉产生的塔拉单宁水解酶活力可达到44.29 U/mL,是其自然条件下酶活力(24.09 U/mL)的1.84倍;没食子酸产率达到79.3%。研究结果对于揭示塔拉单宁生物降解的机理具有一定的参考价值。更多还原

【Abstract】 Vegetable tannins can be degraded into simple phenolic compounds or their derivatives by tannase from microbes.Fermentation conditions have a great effect on production of the hydrolase.Tara tannin hydrolase from Aspergillus niger with the activity of 44.29 U/mL was attained using solid-state fermentation under the conditions of 75 g/L tara tannin,3 g/L glucose,0.2 g/L （NH₄）₂SO₄,5.6%（w/v） PUF,initial pH 5.5,inoculum volume 12%（v/v） at 30 ℃ for 72 h.The results can provide some references for understanding tara tannin biodegradation.更多还原