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高效液相色谱法测定利肺片中五味子甲素的含量
Content Determination of Deoxyschisandrin in Lifei Tablets by HPLC
【摘要】 目的建立利肺片中五味子甲素的含量测定方法。方法采用高效液相色谱法,以C18色谱柱(5μm,4.6 mm×200 mm);甲醇-水(77∶23)为流动相;流速1.0 m l/m in,检测波长为313 nm。结果五味子甲素在0.068~2.72μg范围内呈良好的线性关系,r=0.999 9。平均回收率为99.76%,RSD为0.79%。结论该方法简单、灵敏度高,可用于利肺片中五味子甲素的含量测定。
【Abstract】 Objective To establish an HPLC method for the determination of Deoxyschisandrin in Lifei Tablest.Methods The CenturySIL C18 BDS(200 mm×4.6 mm) was used with methanol-water(77∶23) as a mobile phase.The flow rate was 1.0 ml ·min-1.The detecting wavelength was 313 nm.Results The average recovery was 99.76%.Conclusion The method is simple,rapid and accurate.
【关键词】 利肺片;
五味子甲素;
高效液相色谱;
含量测定;
【Key words】 Lifei tablets; Deoxyschisandrin; HPLC; Quantitative detection;
【Key words】 Lifei tablets; Deoxyschisandrin; HPLC; Quantitative detection;
- 【文献出处】 时珍国医国药 ,LiShiZhen Medicine and Materia Medica Research , 编辑部邮箱 ,2006年12期
- 【分类号】R286
- 【被引频次】3
- 【下载频次】93