【摘要】 目的通过研究尿素水解对内氏放线菌的生长和耐酸能力的影响,了解尿素水解对内氏放线菌的生理作用。方法比较内氏放线菌利用尿素或其它物质作为氮源的生长浊度;采用耐酸实验研究尿素水解与细菌耐酸能力的关系。结果与其他口腔中常见氮源物质相比,尿素可以促进内氏放线菌生长,获得更高的A值;在临床牙菌斑能够检测到的pH4.0~7.0范围内,尿素水解可以提高内氏放线菌的耐酸性,在pH3.0,尿素水解对细菌没有保护作用。结论尿素水解可以促进内氏放线菌生长,提高酸性环境中细菌的耐酸能力,增强细菌的生存竞争力。更多还原

【Abstract】 Objective: To study the effects of ureolysis on the proliferation and acid-resistance of Actinomyces naeslundii.Methods:The growth of Actinomyces naeslundii cultured in different nitrogen sources were measured, and the acid-resistant ability of Actinomyces naeslundii cultured in acid condition with different urea concentration was compared.Results:In contrast with other nitrogen sources,Actinomyces naeslundii could be cultured by urea and higher final A value of the bacteria was obtained;at the range of pH=4.0-7.0, ureolysis could increase the survival of Actinomyces naeslundii from acid killing to some extend, but at pH=3.0, ureolysis could not protect Actinomyces naeslundii anymore.Conclusion:Ureolysis may stimulate the growth of A. naeslundii and increase the acid-resistant ability of Actinomyces naeslundii.更多还原