节点文献
双酶复合法制备大豆多肽工艺的研究
Study on the preparation technology of soy peptides by double-enzyme composite hydrolysis
【摘要】 确定了双酶复合法提取制备大豆多肽的工艺条件。大豆蛋白经双酶复合酶解、活性炭脱色、超滤、真空浓缩和离心喷雾干燥等工艺,提取得到大豆多肽。通过试验确定,最佳复合酶解条件:中性蛋白酶与菠萝蛋白酶比例为3∶1、pH 7.0、底物浓度4.0%、酶解时间9 h;最佳脱色处理条件:粉末活性炭用量2.0%、温度50℃、pH 3.0、吸附时间3 h;超滤分离条件:NF-1纳滤膜、截留分子量2000 D、超滤压力1.0 mPa。
【Abstract】 The preparation technology of soy peptides by double-enzyme composite hydrolysis was studied.Soy protein isolate was produced into soy peptides based on double-enzyme composite hydrolysis and purification technology.The result showed that the best composite enzymatic hydrolysis condition was: the composite enzymes were made up of neutral proteinase and bromelain with the proportion of 3∶1,pH value of 7.0,substrate concentration of 4.0%,hydrolysis time of 9 h;the best decoloration condition was: quantity of activated carbon of 2.0%,temperature of 50℃,pH value of 3.0,adsorption time of 3 h;the ultra-filtration condition was: NF-2 nanometer membrane,pressure of 1.0 mPa.
【Key words】 soy peptides; double-enzyme composite hydrolysis; decoloration; ultra-filtration;
- 【文献出处】 粮油食品科技 ,Science and Technology of Cereals,Oils and Foods , 编辑部邮箱 ,2006年02期
- 【分类号】TS214.2
- 【被引频次】39
- 【下载频次】583