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河南贲门癌高发区贲门癌的组织化学染色分类及P53和PCNA研究
The studies of histochemistry staining patterns and P53, PCNA on gastric cardia adenocarcinoma (GCA) from patients at high-incidence area for GCA in Henan
【摘要】 目的探讨贲门腺癌(Gastriccardia adenocarcinoma,GCA)的组织化学染色分类与P53和PCNA分子学特征。方法采用爱显蓝-雪夫试剂染色法(Alcianblue-Periodicacid Schiff,AB-PAS)和高铁二胺-爱显蓝染色法(High Iron Diamine-Alcianblue,HID-AB)以及ABC免疫组织化学分析(Immunohistochemistry,IHC)法研究了70例贲门癌的组织化学及P53和PCNA蛋白表达变化特征。结果肠型GCA的P53蛋白阳性表达(+~)率(73.08%)明显高于非肠型GCA(29.55%)(P<0.01);PCNA蛋白阳性高表达(~)率(96.15%)也明显高于非肠型GCA(65.91%)(P<0.01)。含硫化粘蛋白的GCA组织中P53蛋白阳性表达(+~)率(70.59%)明显高于不含硫化粘蛋白的GCA(41.51%)(P<0.05);PCNA蛋白阳性高表达(~)率(94.12%)也明显高于不含硫化粘蛋白的GCA(71.70%)(P<0.05)。结论组织化学染色可作为判断GCA不同组织类型和分类的重要指标,并可能对了解GCA临床生物学特征(如预后)具有重要意义。
【Abstract】 Objective: To characterize the histochemistry and expression of P53,PCNA proteins on gastric cardia adenocarcinoma (GCA) .Methods: The methods of Histochemical (AB-PAS and HID-AB methods) and immunohistochemical analysis (ABC method) were performed on the 70 cases of surgically resected tissues with GCA . Results: The positive expression rate for P53 protein (+~ ) in intestinal type GCA (73.08%) was significantly higher than that in non-intestinal type GCA (27.27%,P<0.01), and the high positive expression rate of PCNA protein (~) in intestinal type GCA (96.15%) was significantly higher than that in non-intestinal type GCA (65.91%,P<0.01). The positive expression rate for P53 protein (+~) in sulfuration type (70.59%) was significantly higher than that in non-sulfuration type (41.51%,P<0.05), and the high positive expression rate of PCNA protein (~) in sulfuration type (94.12%) was significantly higher than that in non-sulfuration type (71.70%,P<0.05).Conclusion: The positive expression rate for P53 (+~ ) and PCNA (~ ) in intestinal type GCA were significantly higher than those in non-intestinal type GCA, and in sulfuration type GCA were significantly higher than that in non-sulfuration type GCA, which indicated that histochemical staining may be one of promising biomarkers for GCA classification and histotype, possibly for further understanding of the clinical behavior of GCA (e.g.prognosis).
【Key words】 Esophageal neoplasms/pathology Stomach neoplasms/pathology Gene; P53 Prolifrating cell nuclear antigen;
- 【文献出处】 陕西医学杂志 ,Shaanxi Medical Journal , 编辑部邮箱 ,2006年10期
- 【分类号】R735.2
- 【被引频次】5
- 【下载频次】73