【摘要】 目的:动态观察N_苄基氟哌啶醇氯化物（F3）对大鼠胸主动脉平滑肌细胞内Ca2+的影响。方法:用Ca2+荧光染料Fluo_3AM负载大鼠胸主动脉平滑肌细胞,在激光扫描共聚焦显微镜上测定细胞内游离钙的变化。结果:在含CaCl2（1.26mmol/L）的细胞外液中,30 mmol/L KCl使细胞内钙荧光强度（FI）迅速增强,F3可以拮抗KCl诱导钙FI增强作用,并呈量效依赖和时间依赖关系。4种浓度F3（0.01、0.1、1.0、10μmol/L）作用3 min时,使KCl诱导细胞内钙FI从（81±4）%分别降至（71±18）%（n=20,P<0.05）、（51±12）%（n=20,P<0.01）、（39±14）%（n=20,P<0.01）、（28±14）%（n=20,P<0.01）。钙FI变化最快的时程是在给药后的0³0 s。结论:F3通过阻断细胞膜电压依赖性钙通道降低平滑肌细胞内钙浓度,这可能是其扩张血管,改善心肌缺血的机制。更多还原

【Abstract】 Objective:) To investigate the effects of N-benzyl haloperidol（chloride（F-3）on） intracellular calcium([Ca²⁺]-i)in vascular smooth muscle cells（VSMCs）isolated from thoracic aortas of rat.Methods: VSMCs were loaded with a calcium sensitive fluorescent dye Fluo-3-AM,and [Ca²⁺]-i was determined by the use of laser scanning confocal microscope.Results: In the extracellular solution with Ca²⁺ at the concentration of 1.26 mmol/L,30 mmol/L KCl induced a rapid increase of intracellular calcium fluorescence intensity（FI）.F-3 antagonized the increase of FI induced by KCl in both concentration dependent manner and time dependent manner.After 3 minutes of the administration of F-3 at the concentration of 0.01,0.1,1.0,10 μmol/L,the increased FI of the VSMCs induced by KCl was decreased from（81±4）% to（71±18）%（n=20,P<0.05）,（51±12）%（n=20,P<0.01）,（39±14）%（n=20,P<0.01）,（28±14）%（n=20,P<0.01）,respectively.During 0～30 seconds after the administration of drugs,the changes of FI were more ra-pid.Conclusion: F-3 reduces[Ca²⁺]-i via inhibition of voltage dependent calcium channel,which is possibly involved in the mechanism of its vasodilatory and anti-myocardial ischemic effects.更多还原