【摘要】 目的:研究断奶小鼠在受到微生物刺激时,日粮核苷酸的抗氧化作用,并探讨其可能机制。方法:用脂多糖(LPS)构建氧化应激模型,检测外源核苷酸对小肠组织中谷胱甘肽(GSH)、超氧化歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、一氧化氮(NO)、丙二醛(MDA)、丙氨酸氨基转移酶(ALT)以及髓过氧化物酶(MPO)的变化。结果:在LPS刺激后的4h和18h,日粮核苷酸均能升高肝脏和小肠组织的GSH水平和SOD、GSH-PX活性,降低肠道MDA、MPO含量以及血清ALT。结论:日粮核苷酸有助于增强氧化应激小鼠的抗氧化能力,降低组织NO含量与NO合成酶活性,保护机体免受氧化损伤,外源核苷酸可以减少核苷酸从头合成时与GSH争夺前体物质,增加了GSH水平,进一步增加了SOD、GSH-PX等抗氧化酶的活性。更多还原

【Abstract】 Objective: To study the effects of dietary nucleotides on anti-oxidation and its mechanism on newly weaned mice under stimulation. Method: To determine changes on hepatic and intestinal glutathione (GSH), superoxide dismutase(SOD), glutathione peroxidase(GSH-PX), malondialdehyde(MDA),alanine aminotransferase(ALT)and myeloperoxidase(MPO)by nucleotides free (NF) and nucleotides (NT) diet mice under lipopolysaccharide (LPS) induced oxidative response. Results: Hepatic and intestinal GSH, SOD, GSH-PX increased and MDA decreased with nucleotides supplement 4 and 18 hours after LPS stimulation. Conclusion: Dietary nucleotides can help to increase anti-oxidation, protect mice from injury under LPS stimulation. The mechanism might involve in dietary nucleotides saving glycin, glutamine and energy, which increase GSH and further increase SOD, GSH-PX activity.更多还原