【摘要】 研究了提取及分离纯化大蒜绿变物质的方法。结果表明,浓度为80%的甲醇溶液,按料液比1∶3,室温浸提24h,提取效果最好;利用薄层层析法进行色素的分离纯化,确定展层剂为含77%甲醇,3%乙酸乙酯,0.24%HCl的水溶液,最终能达到初步分离纯化的目的。更多还原

【Abstract】 A method for extraction, isolation and purification of the pigment in greening garlic was determined. The results showed that the best solvent of extracting was 80% CH3OH, the ratio between material to solvent was 1∶3, extraction time was 24h, and that the pigment was isolated and purified by TLC, and the conditions which we have gained were water solution containing 77%CH3OH, 3%CH3COOC2H5 and 0.24%HCl. At last, the pigment could be preliminarily isolated and purified.更多还原