【摘要】 目的探讨BCG对小鼠Th1/Th2细胞免疫反应的调节作用,研究BCG是否增强小鼠Th1型免疫反应。方法将实验动物分为对照Ⅰ组、BCG14组、对照Ⅱ组、BCG42组。BCG14组、BCG42组给予BCG每次0.03 mL,隔日1次腹部皮下注射,对照组注射相同剂量的生理盐水。第14天,将对照Ⅰ组、BCG14组分别处死取脾脏,BCG42组则继续行BCG免疫,至第42天对照Ⅱ组及BCG42组处死取脾脏。用ELISA法测定脾脏单个核细胞(MNCs)培养上清液中IL-4I、L-5I、FN-γ含量。结果BCG14组、BCG42组IFN-γ水平较对照组明显增加(t=3.73、3.86,P<0.01)。BCG14组IFN-γ水平与BCG42组比较差异无显著性意义(t=1.68,P>0.05)。其他两项指标与对照组比较差异无显著性(t=1.05~1.17,P>0.05)。结论BCG能上调小鼠Th1型免疫反应。更多还原

【Abstract】 ObjectiveTo investigate the effect of BCG on the regulation of Th1/Th2 balance in mice and explore whether BCG increased type Th1 immune response.MethodsThe experimental animals were divided into Control Group Ⅰ and BCG14 Group;Control Group Ⅱ and BCG42 Group.For BCG14 Group and BCG42 Group,a dose of BCG 0.03 mL was injected subcutaneously every other day and normal saline,same dose,for control groups.On day 14,those in Control Group Ⅰ and BCG14 Group were killed and spleens removed;those in BCG42 Group continued BCG immunity and killed on day 42,and the spleens removed.The levels of interleukin-4,interleukin-5 and interferon-γ of mononuclear cells(MNCs) supernatant were measured by ELISA.(ResultsIFN-γ) levels in BCG14 and BCG42 groups were significantly higher than those in the control group(t=3.73,3.86;P<0.01).The difference of IFN-γ levels of MNCs supernatant between BCG14 and BCG42 was not significant(t=1.68,(P>)0.05).As compared with control groups,the difference of the other two items showed no significance(t=1.05-1.17,P>(0.05)).ConclusionBCG immunization could up-regulate Th1-type immune response.更多还原