【摘要】 目的为改善天然抗氧化物质抗氧化活性单一的不足,研究有效的天然产物配方。方法选取3种天然植物抗氧化物质芦丁、大豆异黄酮、姜黄素,利用正交试验设计选择合适配方,以自由基DPPH的清除率作为正交试验评价标准,并利用人肝肿瘤细胞SMMU-7721进行四甲基偶氮唑盐(MTT)试验来考察配方体外试验对肿瘤细胞的抑制作用。结果最终得到芦丁、大豆异黄酮、姜黄素配方比例为2∶3∶7,MTT试验结果与DPPH清除试验均显示配方效果高于单方物质。结论配方比单方成分有更好的抗氧化能力,正交试验所得的比例较为合适。更多还原

【Abstract】 PurposeTo cut down production cost and to make improvement of monofactorial natural antioxidant.MethodsThe article applied the orthogonal design to find out the optimum proportion of the rutin,soybean isoflavone and curcumin.The free radical DPPH· scaving ratio was used as evaluating standard of orthogonol design and the hominal liver cancer cell SMMU-7721 was used in MTT trial to evaluate the inhibitory ability of the compound.ResultsThe optimum proportion of rutin-soybean isoflavone-curcumin 2∶3∶7 was achieved.MTT trial got the simlar conclusion to the DPPH scaving trial.This meant that the compound has better activity than any one monofactor.ConclusionWe can conclude that the compound is more active than its component and the optimum proportion is 2∶3∶7.更多还原