【摘要】 目的:构建人S-腺苷蛋氨酸脱羧酶(SAMDC)的α亚基的原核表达载体,诱导该质粒在大肠杆菌中表达并纯化表达的重组蛋白。方法:从大肠癌细胞中提取总RNA,RT-PCR方法扩增人SAMDCα亚基的cDNA片段801 bp,经TA克隆及亚克隆方法构建原核表达载体pTriEx-4-SAMDC-α。将重组表达质粒转化入E.coliJM109(DE3)中,经IPTG诱导表达,SDS-PAGE电泳和Western blot鉴定表达蛋白,并通过6×His.Tag,利用亲和层析法纯化表达的融合蛋白。结果:酶切鉴定和DNA测序显示,人SAMDCα亚基的cDNA片段成功插入表达载体pTriEx-4且方向正确,SDS-PAGE电泳显示表达出32kD的外源蛋白。Western blot检测结果显示,表达出的蛋白为6×His.Tag的融合蛋白,且Ni-NTA亲和层析法纯化了该重组蛋白。结论:成功构建、表达且纯化了重组SAMDC-α亚基,为制备抗SAMDC抗体、研究SAMDC基因与结直肠肿瘤的关系提供了必要的工具。更多还原

【Abstract】 Objective: To construct the prokaryotic expression plasmid of human S-adenosylmethionine decarboxylase(SAMDC) α subunit,and to induce and purify the recombined SAMDC-α protein.Methods: A 801?bp cDNA spanning the coding region of SAMDC α subunit was amplified by RT-PCR and subcloned into prokaryotic expression vector pTriEx-4.The resulted vector pTriEx-4-SAMDCα was transformed into competence E.coli JM109(DE3),then was induced by IPTG.The protein was verified by SDS-PAGE and Western blot with anti His·Tag monoclonal antibody.The fusion protein including 6×His·Tag was purified by Ni-NTA chromatographic column.Results: A 801?bp cDNA was successfully amplified by RT-PCR from colorectal cancer cells and confirmed by sequence.The prokaryotic expression plasmid pTriEx-4-SAMDC-α was successfully constructed,which was identified by digestion and sequence.An approximate 32kDa exogenous protein was observed on the SDS-PAGE and Western blot showed that the protein including 6×His·Tag was purified successfully by Ni-NTA affinity chromatography.Conclusion: The prokaryotic expression plasmid pTriEx-4-SAMDC-α is constructed correctly and the fusion protein is expressed and purified successfully.更多还原