【摘要】 目的:观察外周血免疫调节性T细胞(Treg)在感染后肠易激综合征(PI-IBS)大鼠模型中的变化,探讨Treg在其发病机制中的作用。方法:随机将16只大鼠分为模型组和正常对照组,分别用福氏IV型志贺氏痢疾杆菌和生理盐水灌胃。模型组急性肠道感染16~22 d,测定两组大鼠的肠道感觉阈值和平滑肌张力积分,远端结肠行病理学检查,用流式细胞仪分析外周血Treg的比例。结果:模型组肠道感觉阈值明显降低(P<0.05),肠道平滑肌张力积分明显升高(P<0.05),而组织学无明显变化,表明PI-IBS大鼠模型建立成功;模型组外周血Treg/CD4+T比值明显下降(P<0.05)。结论:大鼠在急性肠道感染消失后,会出现PI-IBS;免疫调节的异常参与了PI-IBS的发病过程。更多还原

【Abstract】 Objective: To observe the changes of regulatory T cells(Treg cells) in peripheral blood in rat model with post infectious irritable bowel syndrome(PI-IBS) and investigate the role of Treg cells in its pathogenesis.Methods: A total of 16 female rats were divided into model and control groups,who were given shigella flexneri by gavage and saline by gavage,respectively.After the bowel inflammation had been induced for 16～22 days,all rats were treated with colon-rectal balloon distention;the contraction of enteric smooth muscle were measured in vitro and the proportion of Treg cells in peripheral blood was detected by using flow cytometry analysis.Results: In model group,the perceptual threshold decreased and the tension integral of enteric smooth muscle increased greatly compared with the results from control group(P<0.05),while no obvious histological changes had been observed, suggesting rat model of PI-IBS had been successfully established;the value of Treg/CD4~+T in peripheral blood decreased significantly compared with the result from control group(P<0.05).Conclusion: Bowel functional disorder is prolonged after acute enteric inflammation has disappeared.Abnormal immunoregulation takes account for the pathogenesis of PI-IBS.更多还原