【摘要】 目的测定悬浮培养金丝桃细胞中金丝桃苷的含量。方法采用高效液相色谱法,色谱柱为HypersilODS2-Cl8柱(4.6mm×250mm,5),0.5%磷酸-甲醇为流动相,采用梯度洗脱,柱温30℃,流速为1.0mLin-1,紫外检测波长为358nm。结果线性范围为0.174～1.740μg(r=0.9998),平均回收率为99.25%,RSD=1.28%(n=6)。结论本法简便、准确和重复性好,可作为工厂化生产金丝桃药材细胞质量控制的有效方法。更多还原

【Abstract】 Objective To establish a HPLC method for the determination of the content of hyperoside insuspension culture of Hypericum perforatum L.cells.Methods The separation was performed on Hypersil ODS2-C18 column(4.6 50mm,5) with a gradient mobile phase consisting of a mixture of 0.5% phosphoric acid-methanol.The column temperature is 30℃,the flow rate was 1.0 mLin-1 and the detective wavelength was 358 nm.ResultsThe linearity of the method was well within the range from 0.174 to 1.740μg(r=0.999 8) and the average recoveryof hyperoside was 99.25% with RSD=1.28%(n=6).Conclusion The method is simple,accurate and reliable,soit can be used for the quality control of Hypericum perforatum L.cells production in factories.更多还原