【摘要】 目的研究上皮性卵巢癌组织中P-糖蛋白、Survivin蛋白和拓扑异构酶Ⅱ的表达及其临床意义。方法采用免疫组化法检测34例卵巢上皮性癌、25例卵巢良性上皮性肿瘤和20例正常卵巢组织P-糖蛋白、Survivin蛋白和拓扑异构酶Ⅱ的表达。结果卵巢癌组织中P-糖蛋白、Survivin蛋白和拓扑异构酶Ⅱ的阳性表达率分别为29.41%(10/34)、64.71%(22/34)及50.00%(17/34),正常卵巢组织及卵巢良性上皮性肿瘤中P-糖蛋白、Survivin蛋白均未表达,拓扑异构酶Ⅱ均为阳性表达。卵巢癌组织中P-糖蛋白阳性表达率在各组织类型之间差异极显著(P<0.01),其中卵巢黏液性囊腺癌阳性表达率最高为66.67%(4/6);Survivin蛋白、拓扑异构酶Ⅱ的阳性表达率在各组织类型间差异无显著性(P>0.05);P-糖蛋白、Survivin蛋白、拓扑异构酶Ⅱ在卵巢上皮性癌的表达与病理分级及临床分期均无明显相关性(P>0.05)。结论联合检测P-糖蛋白、Survivin蛋白和拓扑异构酶Ⅱ的阳性表达率对卵巢癌化疗药物的选择具有指导意义,对预后判断有重要参考价值。更多还原

【Abstract】 Objective To explore the expression of P-Glycoprotein,survivin protein and topoisomeraseⅡ(TopoⅡ)in ovarian epithelial cancer tissues and its clinical significance.Methods Immunohistochemistry was used for determination of the expression of P-gp,survivin protein and topoⅡin 34 ovarian epithelial carcinoma cases,25 epithelial benign ovarian tumors cases and 20 normal ovarian tissue cases.Results The positive expression rates of P-gp、survivin protein and topoⅡ in ovarian epithelial cancer tissues were 29.41%(10/34)、64.71%(22/34)and 50.00%(17/34),respectively.P-gp and survivin protein were negtive in normal ovarian tissues cases and epithelial benign ovarian tumors cases,while topoⅡ was positive in these two tissues.There was a significant difference among the three histological types of P-gp expression rate in ovarian epithelial cancer tissues(P<0.01),that of mucinous cystodenocarcinoma was the highest,was 66.67%(4/6).There was no sgnificnt difference in expression of survivin protein and topoⅡ in different histological types(P>0.05).There were non significant difference in expression rates of P-gp,survivin protein and topoⅡ in epithelial ovarian cancer tissues at various tumor pathological grade and clinical stage(P>0.05).Conclusion Joint determination of P-gp,survivin protein and topoⅡ is valuable for selecting drugs and the estimation of the consequence.更多还原