【摘要】 采用高效液相色谱法,建立了油菜植株和土壤中多菌灵残留量的检测方法。样品经乙酸乙酯提取,AMINOSPE柱净化和浓缩后,C18柱进行分析。流动相为甲醇∶水=85∶15、流速为1.0ml/min、检测波长280nm时,方法的最低检出限为0.01mg/L,并用于油菜植株和土壤实际样品中多菌灵残留量的检测及动态分析。田间降解动态试验结果表明,多菌灵在油菜植株中较土壤中缓慢,半衰期大约32d。更多还原

【Abstract】 A high performance liquid chromatography method was developed for determining carbendazim residues inrapeseed plants and soil. Samples were extracted with ethyl acetate and purified on an AMINO solid phase extractioncolumn. Determination was conducted with HPLC using a 250 × 4.6 mm C18 column, and a mobile phase ofmethanol-water (85:15). Detection wavelength was 280 nm and flow rate of the mobile phase was 1.0 ml/min. Thelower limit of detection was 0.01 mg/L. This method was also used to determine the levels and dynamics of carbendazimresidues in actual rapeseed and soil samples. Field dissipation dynamics experiments indicated that carbendazimdissipated more slowly in rapeseed plants than in soil, with the half-life in rapeseed being 32 d.更多还原