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芪合酶基因转化小白菜

Transgenic Plants of Brassica campestris with Resveratrol Synthase Gene

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【Author】 HU Xiao-ping1,2, SHANG Wen-jing1, CAI Wen-qi2, HAN Qing-mei3, KANG Zhen-sheng1,3 (1. College of Plant Protection, Northwest Agriculture and Forestry University, Yangling 712100, China; 2. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China; 3. Key Laboratory of Agricultural Molecular Biology in Shaanxi Province, Northwest Agriculture and Forestry University, Yangling 712100 , China)

【机构】西北农林科技大学植物保护学院；中国科学院微生物研究所；西北农林科技大学陕西省农业分子生物学重点实验室；西北农林科技大学植物保护学院杨陵712100 中国科学院微生物研究所；北京100080；杨陵712100；杨凌712100；杨陵712100 西北农林科技大学陕西省农业分子生物学重点实验室；

【摘要】利用质粒pBin438构建了含有NPTⅡ基因和葡萄芪合酶(RS)基因的芪合酶组成型植物表达载体EHA105/pBinRS。用根癌农杆菌(Agrobacteriumtumefaciens)介导法将RS基因导入纯合系小白菜(Brassicacampestrisssp.chinensis)品种中,经Kan抗性筛选、PCR及RT-PCR检测,RS基因已整合到小白菜基因组中并得到了表达,共获得了7株转基因植株。更多还原

【Abstract】 A plant constitute expression vector EHA105/pBinRS, which contained NPTⅡ and resveratrol synthase (RS) gene, was constructed through plasmid pBin438. RS gene was then transferred into Brassica campestris ssp. chinensis using an Agrobacteriume tume faciens mediated method. The regeneration plants were screened on MSBk medium and assayed by PCR and RT-PCR. The results showed that RS gene had been integrated into genome of B. campestris ssp. chinensis and normally expressed. And seven transgenic plants were obtained.更多还原

【关键词】小白菜；转基因植株；芪合酶基因；
【Key words】 Brassica campestris； transgenic plant； resveratrol synthase gene；

【基金】陕西省农业分子生物学重点实验室开放基金;西北农林科技大学创新团队资助项目

【文献出处】农业生物技术学报 ,Journal of Agricultural Biotechnology , 编辑部邮箱 ,2006年02期
【分类号】S634.3
【被引频次】6
【下载频次】131

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