【摘要】 研究了采用酶解法提取啤酒糟中蛋白质的工艺条件,通过正交试验得到提取的最佳工艺条件为:水解蛋白酶的添加量2mL/100g干啤酒糟,反应温度60℃,pH8.0,反应时间5h,固液比11∶2。在此条件下,水解蛋白提取率为63.6%。采用高效液相法对酶解液中的18种氨基酸含量进行了分析。18种游离氨基酸含量占总蛋白含量的24%,8种游离状态的必需氨基酸占游离氨基酸的39%。更多还原

【Abstract】 The optimum conditions of enzymatic hydrolysis of brewer’s spent grains was studied.The optimum conditions: the enzyme load is 2ml/100g dried brewer’s spent grains;the temperature of hydrolysis is 60℃;the pH of hydrolysis is 8.0;the time of hydrolysis is 5 hour;the proportion of solid and liquid is 1:12.At the optimum conditions,the extraction rate of protein could reach 63.61%.18 kinds of amino acid in liquid were determined by HPLC.The content of 18 kinds of free amino acid is 24% by which of total protein.The content of 8 kinds of amino acid that can’t be synthesized in human’s body is 39% by which of free amino acids更多还原