【摘要】 对60Co诱变后得到的高产酒精酵母菌株Co-158进行了发酵工艺优化研究。通过正交试验确定其适宜发酵工艺条件为:料水比12∶,接种量4.5%,发酵时间84h,初始pH值4.5;对流加葡萄糖工艺进行优化,适宜工艺为:以玉米粉糖化醪为基质,发酵时间84h,发酵过程中间歇流加6%的葡萄糖,其三角瓶发酵酒精产率为19.20%(v/v),残还原糖为0.685%;10L发酵罐小试酒精产率为18.60%(v/v),残还原糖为0.013%。更多还原

【Abstract】 The fermentation technology of high-yield alcohol yeast strain Co-158(produced by the mutagenesis of 60Co) were optimized and the best technical conditions in fermentation were determined by orthogonal test as follows: the ratio of raw materials and water was 1∶2,4.5 % inoculation quantity,84 h fermentation time,and initial pH value was 4.5.Besides,glucose addition techniques were optimized as follows: corn flour sweet mash used as ground substance,84 h fermentation time,addition of 6 % glucose at intermittent flow in fermentation,alcohol yield by triangle flask fermentation was 19.20 %(v/v) and the content of residual reducing sugar was 0.685 %,and alcohol yield by 10 L fermenting pot fermentation was 18.60 %(v/v) and the content of residual reducing sugar was 0.013 %.更多还原