【摘要】 将栽培种花生8823体细胞胚和匍匐区组近缘野生种A.rigonii幼茎分离得到的原生质体用PEG方法融合后,培养在添加1mg/L NAA和6mg/L BAP的改良MSB5培养基中进行液体浅层培养或固液双层培养。3d后开始观察到细胞分裂,之后细胞继续分裂生长。培养7~8周后,形成直径1~2mm的愈伤组织。固液双层培养优于液体浅层培养,其细胞置板率高且不易聚集。更多还原

【Abstract】 Protoplasts isolated from somatic embryos of peanut cv.8823 and slender stem of A.rigonii were fused by PEG method.The fusion products were cultured in a modified MSB5 medium containing 1mg/L NAA and 6mg/L BAP.The first cell division occurred within 3 days.Then some of the cells divided and developed into colonies.After 7 to 8 weeks,the small calli grew up to 1-2 mm in diameter.更多还原