【摘要】 目的探讨非小细胞肺癌(non-sm all cell lung cancer,NSCLC))急慢性乏氧状况、乏氧与COX-2过度表达及与临床病理特征的关系。方法应用兔抗人H IF-1α、GLUT1抗体和鼠抗人COX-2抗体对手术切除的46例肺癌组织进行免疫组化染色,并与临床病理资料进行相关性分析。结果肺癌患者46例,其中ⅠA期12例,ⅠB期20例,Ⅱ期8例,ⅢA期6例。H IF-1α、GLUT1和COX-2的阳性表达率分别为58.70%(27/46)、39.11%(18/46)和67.39%(31/46)。COX-2表达与H IF-1α、GLUT1的表达相关(P<0.01)。GLUT1表达与肺癌患者淋巴结转移(P<0.05)、组织学类型(P<0.01)有关,而H IF-1α、COX-2表达与年龄、性别、分期、肿瘤大小、淋巴结转移、组织学类型和肿瘤分化程度均无相关性。结论NSCLC患者同时存在着急、慢性乏氧,而肿瘤转移主要与慢性乏氧有关。另外,乏氧可能参与了COX-2增强表达的机制。更多还原

【Abstract】 Purpose To investigate the expression of HIF-1α and GLUT1 in NSCLC which are considered as acute and chronic hypoxic markers respectively,and their potential roles in the over-expression of COX-2 and correlation with clinicopathological characteristics.Methods Immunohistochemistry was used to detect the expression of HIF-1α,GLUT1 and COX-2 in sequential histologic sections of specimens from 46 cases of primary lung carcinomas.Results HIF-1α,GLUT1 and COX-2 stains were observed in 58.70%(27/46),39.11%(18/46) and 67.39%(31/46) of the cancers respectively.COX-2 expression was closely related to both HIF-1α and GLUT1(P<0.01).GLUT1 expression was significantly correlated with lymph node metastasis(P<0.05) and squamous cell histology(P<0.01).Neither COX-2 nor HIF-1α expression was closely related to clinico-pathological characteristics,such as age,sex,pathological stage,tumor size,lymph node metastasis,histology,and grade of differentiation.Conclusions Both acute and chronic hypoxia exist in lung cancer tissues.GLUT1 over-expression other than HIF-1α is related to lymph node metastasis.Furthermore,hypoxia may play a role in the mechanism of COX-2 over-expression.更多还原