【摘要】 目的:探讨声门上喉癌、下咽癌瘤体、周围组织及转移淋巴结真核细胞翻译起始因子4E(eIF4E)、基质金属蛋白酶-9(MMP-9)的表达情况,以及2种分子标志物表达的相关性。方法:采用免疫组织化学SP法及图像分析,检测38例肿瘤瘤体及不同距离手术切缘组织、转移淋巴结中eIF4E、MMP-9的表达情况。结果:①在喉及下咽部鳞状细胞癌组织中MMP-9阳性表达率为68.4%,表达强度与淋巴结转移、临床分期相关,而与肿瘤位置及病理分级无关。MMP-9在声门上喉癌、下咽癌瘤体及5mm、10mm切缘组织中的表达阳性率依次递减;②喉及下咽部鳞癌组织eIF4E表达阳性率为89%,表达强度与淋巴结转移、肿瘤病理分级、临床分期相关。eIF4E在声门上喉癌、下咽癌瘤体及5mm、10mm切缘组织表达阳性率依次递减;③MMP-9和eIF4E在正常淋巴结中均无表达,在转移淋巴结表达阳性率分别为28%和42%;④eIF4E与MMP-9表达无相关性。结论:eIF4E、MMP-9在喉癌及下咽癌瘤体中的表达高于癌周正常组织,eIF4E在瘤体及癌周正常组织表达阳性率高于MMP-9。eIF4E可作为预测喉癌及下咽癌浸润生长、转移的敏感标志物。更多还原

【Abstract】 Objective:To study the expression of eukaryotic initiation factor4E（eIF4E）and matrix metalloproteinase-9（MMP-9） in supraglottic,hypopharyngeal carcinoma,different distance surgical margins and lymphatic metastasis; to study the relationship of two molecular markers.Method:Expression of eIF4E and MMP-9 in carcinoma and different margins were detected by HE-stained, routine pathological section, immuohistochemical SP method and image analysis.Result: Immunohistochemical staining for MMP-9 was performed with a monoclonal anti-MMP-9 antibody. The positive expression rate was 68.4%.There was significant difference between lymph node metastasis and no lymph node metastasis ,but there was no significant difference between T₃ and T₄ or T₁ and T₂（P>0.05）.There was no significant difference between MMP-9 and carcinoma position,pathology grade（P>0.05）.Positive expression rate of MMP-9 was decreased in tumour , 5 mm, 10 mm different distant margin of supraglottic and hypopharyngeal carcinoma as tissue progressed adjacent to carcinoma.②Staining positive rate of eIF4E in supraglottic and hypopharyngeal carcinoma was 89%. There was significant association between eIF4E expression intensity and clinical stage, lymphic metastasis. The positive expression rate of eIF4E was increased in sequence from 10 mm, 5 mm distant mucosa tissue to carcinoma nest in supraglottic and hypopharyngeal carcinoma.③Negative expression of MMP-9 and eIF4E was seen in the normal lymph nodes. MMP-9 positive expression rate was 28% in metastasic lymph nodes,eIF4E was 42%. ④ There was no significant correlation between eIF4E and MMP-9 in margins according to spearman rank correlation test.Conclusion:eIF4E and MMP-9 expression was significantly higher in supraglottic and hypopharyngeal carcinoma than distant margin and normal mucosa tissue. eIF4E positive rate was higher than MMP-9,expression sensitivity and reliability of eIF4E in margins was higher than MMP-9. eIF4E is a sensitive marker in growth, infiltration and metastasis of supraglottic and hypopharyngeal carcinoma.更多还原