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茶树EST资源中SSR的信息分析
Analysis of SSR Information in EST Resource of Tea Plants(Camellia sinensis)
【摘要】 从NCBI公共数据库下载获得1989条茶树EST,去除其中的低质量的和冗余的序列,得到全长为734.54 kb的1589条无冗余EST。共在这些序列中发掘出了281个EST-SSR,分布于246条EST中,出现频率是17.68%。这些EST-SSR的平均长度为33.06 bp,平均分布频率是1/2.61 kb。茶树EST—SSR中,二核苷酸重复是主要的重复类型,出现最多的重复基元类型是AG/CT重复。本研究为茶树EST-SSR标记的建立和进一步应用奠定了基础。
【Abstract】 1989 ESTs of tea plants were downloaded from the public database of NCBI and some of them redundant or with low quality were removed,finally resulting in 1589 non-redundant ESTs with total length of 734.54 kb.Totally 281 SSRs distributed in 246 ESTs were mined out,accounting for 17.68% of the non-redundant ESTs.The average length of tea plants EST-SSRs searched is 33.06 bp and the average distance of distribution is 1/2.16 kb.The dinueleotide repeat is the dominant type with repeat motif AG/CT being the most common.The result in this paper provides a base for the development and future application of EST-SSR markers in tea plants.
- 【文献出处】 科技通报 ,Bulletin of Science and Technology , 编辑部邮箱 ,2006年04期
- 【分类号】S571.1
- 【被引频次】33
- 【下载频次】222