【摘要】 目的研究补肾益气生精中药对去卵巢(OVX)大鼠骨组织中信号转导分子Smad2的表达,以及在骨质疏松症发病中的作用机制。方法用去卵巢的方法建立绝经后骨质疏松症模型,给予补肾益气生精中药防治12周,以尼尔雌醇为阳性对照,用双能-X线骨密度仪检测大鼠离体股骨骨密度,用逆转录多聚酶链反应(RT-PCR)、W estern blot方法分别检测Smad2的mRNA、蛋白表达。结果卵巢切除大鼠离体股骨骨密度显著下降,骨组织中Smad2的蛋白及mRNA表达水平均下降。补肾益气生精中药能够提高去卵巢大鼠的骨密度,并且可以从蛋白及mRNA水平提高骨组织中Smad2的表达。结论骨组织中信号转导分子Smad2表达水平的下调可能是原发性骨质疏松症发生的重要机制,补肾益气生精中药通过上调Smad2的表达而有效改善骨密度。更多还原

【Abstract】 Objective To investigate the regulative effect of some Chinese herbal drugs(with the effects of reinforce kidney,replenishing qi and strengthening essence) on the expression of signal transduction(molecules)-Smad2 in the bone tissue of rats after ovariectomy and the mechanism of osteoporosis occurrence.Methods The model of postmenopausal osteoporosis was established through ovariectomy in rats and then treated with Chinese herbal drugs (with the effects of reinforce kidney,replenishing qi and strengthening essence) for 12 weeks.The control group was treated with nilestriol.The bone mineral density(BMD) of rat femoral bone in vitro was detected by dual-energy X-ray densitometer and the expressions of Smad2(protein) and mRNA were detected by reverse transcription-polymerase chain reaction(RT-PCR) and(Western) blotting method respectively.Results BMD of femoral bone in vitro in rats after ovariectomy(decreased) obviously and the expressions of Smad2 protein and mRNA in bone tissue decreased averagely.The Chinese herbal drugs(with the effects of reinforce kidney,replenishing qi and strengthening essence) could increase BMD in rats after ovariectomy and improve Smad2 expression in bone tissue at the levels of protein and mRNA.Conclusion The decrease of Smad2 expression in bone tissue maybe the mechanism of primary osteoporosis.The Chinese herbal drugs(with the effects of reinforce kidney,replenishing qi and strengthening essence) can improve BMD through increasing Smad2 expression.更多还原