【摘要】 8只泌乳期的健康睢宁白山羊,产后72 h分别用内毒素(LPS)和灭菌生理盐水经乳头管灌注左右侧乳房。于灌注后0、3、6、9、24 h分别采集颈静脉血和左右两侧乳房的乳汁,分析相关酶活性和细胞因子含量。结果表明:在实验乳腺(灌注LPS)的乳中N-乙酰-β-D-氨基葡萄糖苷酶(NAG ase)、碱性磷酸酶(ALP)活性在24 h内持续上升,髓过氧化物酶(M PO)活性先上升后下降;而在血清中NAG ase、M PO活性先下降后上升,ALP活性在24 h内持续下降。TNFα-、IL-1、IL-6细胞因子水平在实验乳腺的乳中先上升后下降,S IgA水平在24 h内持续上升;血清中这些细胞因子也是先上升后下降;而灌注生理盐水的对照乳腺的乳中酶和细胞因子水平均无显著变化。更多还原

【Abstract】 Eight healthy lactating Suining goats were intramammary infused in left udder with lipopolysaccharide and the right udder with saline(9 g·L^-1) serving as control.Jugular vein blood and milk of both udders were taken immediately before and at 3,6,9 and 24 h after infusion and correlated enzymes and cytokines were measured to observe their changes.In LPS-treated udder,N-acetyl-β-D-glucosaminidase（NAGase）,myeloperoxidase（MPO） and alkaline phosphatase（ALP） activity increased and peaked at 24,9 and 24 h respectively;however in serum these three enzymes decreased and lowered at 9,9 and 24 h respectively.TNF-α,IL-1,IL-6,SIgA concentration in LPS treated udder increased and peaked at 6,9,9 and 24 h respectively;while these cytokines also increased in serum and they peaked at 6,6 and 9 h respectively.In control udder,enzymes and cytokines had no remarkable change.更多还原