【摘要】 将微小隐孢子虫Cpgp4015基因定向克隆到真核表达载体pVAX1中多克隆位点XbaⅠ和PstⅠ之间,经酶切鉴定,构建真核表达重组质粒pVAX1-Cpgp4015,用脂质体介导法将其转染Hela细胞,并用G418加压筛选,后经SDSPAGE和Westernblot方法检测外源基因Cpgp4015的表达情况,得到预期大小的蛋白带,表明该重组质粒能够在哺乳动物细胞中较好地表达。更多还原

【Abstract】 Cpgp40/15 gene from C.parvum was inserted into eukaryotic expression vector pVAX1 in PstⅠ and XbaⅠ site to construct a recombinant plasmids pVAX1-Cpgp40/15.After transfected into Hela cells by liposomes,expression cell strain were selected with G418.The specific recombinant proteins were detected by SDS-PAGE and Westen-blot methods and protected protein strain obtained,which showed that the recombinant plasmids pVAX1-Cpgp40/15 can highly expressed the protected antigens of C.parvum in Hela cell.更多还原