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微流控芯片分离效果光电检测方法研究

Study on the Photoelectric Technique on Testing the Separation Effect by Microfluidic Chip

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【作者】 刘岩左春柽陈洪利姜立标李月强

【Author】 LIU Yan~(1,2),ZUO Chun-cheng~1,CHEN Hong-li~1,JIANG Libiao~3,LI Yue-qiang~3(1.College of Mechanical Science and Engineering,Jilin University,Changchun 130025,China;2.Shandong Institute of Business and Technology,Yantai 264005,China;3.Weihai Campus,Harbin Institute of Technology,Harbin 264200,China)

【机构】 吉林大学机械科学与工程学院哈尔滨工业大学威海分校哈尔滨工业大学威海分校 吉林长春130025山东工商学院信息与电子学院山东烟台264005吉林长春130025山东威海264200

【摘要】 设计了一套用于微流控芯片分离效果检验的光学检测系统,系统使用生物落射荧光显微镜作为主要光路系统,用彩色CCD摄像头采集图像信息输入计算机,用线形灰度变换算法处理RGB24位彩色图像数据.用该系统对瓢虫翅膀、洋葱细胞、机油、聚氯乙稀颗粒和熔融玻璃管做了检测实验,分析了影响检测效果的因素.

【Abstract】 A microscope detecting system has been developed to detect the separating in electrophoresis chips.An epifluorescent microscope is selected as the main optical system,in which the analog images are collected by the color CCD camera and imported into computer after transformed into digital image by a video adapter card.Then the image process begins by the software developed by the authors.The 24bit RGB image data are conduced by means of line-gray transforming method.The system has been applied to detect ladybug’s wing,onion lice,machine oil,PVC particles and frit micro-tube.Besides,the factors which affect the detecting result have been analyzed.

【关键词】 微流控芯片光学检测CCD图像处理
【Key words】 microfluidic chipoptical testCCDimage process
【基金】 教育部博士基金,编号:20040183057
  • 【文献出处】 佳木斯大学学报(自然科学版) ,Journal of Jiamusi University(Natural Science Edition) , 编辑部邮箱 ,2006年02期
  • 【分类号】TN492
  • 【下载频次】194
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