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重组Tgo DNA聚合酶最适扩增条件的研究

Study on the Optimum Amplification Conditions for Recombined Tgo DNA Polymerase

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【Author】 SHENG Yan-min~(1,5),WU Ying-jie~2,LIAO Jun-jie~3,FENG Bo~(4,5),LI Yan-fang~5(1.Department of Biology,Changchun Teacher′s College,Changchun 130032,China;2.Department of Medicine,Mount Xi′nai School of Medicine,NY 10029,USA;3.Department of Foodstuff and Biotechnology,Guangzhou Light Industry Vocational Technique College,Guangzhou 510300,China;4.Beijing Economic Management School,Beijing 100036,China;(5.College) of Plant Science,Jilin University,Changchun 130023,China)

【机构】长春师范学院生物系；西奈山医学院医学系；广州轻工职业技术学院食品与生物工程系；北京市经济管理学校；吉林大学植物科学学院长春130032；吉林大学植物科学学院；长春130062；美国纽约NY10029；广州510300；北京100032；

【摘要】以重组TgoDNA聚合酶为研究对象,检测了其最适扩增条件。结果表明:该重组酶的最适pH为8.75,最适Mg2+浓度为2.0 mmol/L,最适延伸温度为71.9℃。利用该重组酶成功扩增了质粒pUC19、野大麦Na+/H+逆向运转蛋白基因、拟南芥Timeless基因、小鼠Cx37基因和人CYT2C9基因。更多还原

【Abstract】 The optimum amplification conditions of the recombined Tgo DNA polymerase were examined in this study.The results revealed that the optimum PH of the enzyme was 8.75,the optimum concentration of Mg²⁺ was 2.0 mmol/L,and the optimum elongating temperature was 71.9 °C.Plasmid pUC19, Na⁺/H⁺ reversed transporting protein gene of wild barley,Arabidopsis Timeless gene,mouse Cx37 gene and human CYT2C9 gene were amplified successfully using this recombined enzyme.更多还原

【关键词】重组Tgo酶； pH值； Mg2+浓度；延伸温度；
【Key words】 recombined Tgo enzyme； pH value； Mg²⁺ concentration； elongating temperature；

【基金】吉林省教育厅资助项目(20062200135)

【文献出处】吉林农业大学学报 ,Journal of Jilin Agricultural University , 编辑部邮箱 ,2006年03期
【分类号】Q78
【被引频次】4
【下载频次】102

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