【摘要】 辞腈酶是用途较广的一类酶,在植物体内抵御微生物侵袭方面起着重要作用。在化学工业,醇腈酶用于合成手性氰醇的生物催化剂。氰醇是合成β-氨基醇类,α-羟基酮类及酸类化合物的重要生物活性物质,这些化合物又是精细化工、制药和农用化学品领域的重要原料和中间体。研究人员已经利用蛋白质工程和定点突变技术来改变各种醇腈酶的活性位点和底物特异性等。许多重组醇腈酶业已在大肠杆菌,啤酒酵母和甲醇酵母中成功表达。更多还原

【Abstract】 Hydroxynitrile lyases are the versatile group of enzymes,which play a significant defensive role in plant system against microbial attack. In chemical industries, hydroxynitrile lyase is used as an important industrial biocatalyst for the synthesis of chiral cyanohydrins by exploiting the reversible enzymatic reaction. Cyanohydrins are biologically active compounds used in synthesis of β-amino alcohols, a-hydroxy ketones anda-hydroxy acids, which have importance as fine chemicals, Pharmaceuticals and agrochemicals. Protein engineering and site directed mutagenesis have been used to change the active site and alter the substrate specificity of various ydroxynitrile lyases. Many recombinant hydroxynitrile lyases have been expressed in Escherichia coli, Saccharomyces cerevisiae and Pichia pastoris.更多还原