【摘要】 目的:探讨ALA—PDT对结肠癌细胞增殖抑制及以及对P21c、yclinDl、CDK2的影响。方法:采用MTT法检测PDT后对细胞增殖抑制情况。应用RT—PCR技术检测细胞P21、cyclinDl、CDK2mRNA的含量。结果:PDT后P21mRNA含量较PDT前提高(P<0.01),而cyclinDl、CDK2mRNA的含量下降(P<0.01),SW480细胞出现明显的增殖抑制。结论:ALA—PDT对肿瘤细胞的增殖抑制作用与其诱导P21的过表达,进而下调cyclinDl、CDK2的表达有关。更多还原

【Abstract】 Objective:To study the effect of ALA-PDT and the role of P21,cyclinD1,CDK2 on proliferation of colon carcinoma SW480 cells.Methods:SW480 cells were treated with ALA-PDT.The proliferation of SW480 cells was detected by MTT assay.The expression of P21、cyclinDl,CDK2 mRNA were detected by reverse transcriptase-polymerase chain reaction(RT-PCR)technique.Results: When treated with ALA-PDT,the expression of P21 mRNA in SW480 cells increased remarkably,and the expression of cyclinD1,CDK2 in SW480 Cells decreased compared with the control group(P<0.01).The ability of cell proliferation decreased significantly in SW480 cell line after PDT.Conclusion: The expression of P21,which up-regulated by ALA-PDT induced the down-regulation of cyclinD1 and CDK2,and caused the proliferation inhibition of the colon carcinoma SW480 cell line.更多还原