【摘要】 目的检测PIG11(p53-induced gene 11)蛋白在人体正常组织及其相应肿瘤组织中的表达情况,探讨PIG11蛋白在人体正常组织的分布特点,及在肿瘤发生发展中所起的作用。方法利用免疫组化SP法检测PIG11蛋白在319例人体正常组织及其相应肿瘤组织中的表达。结果PIG11蛋白在胃、肠、肝、乳腺等腺上皮及鳞状上皮组织中明显表达阳性,在正常胃黏膜腺颈部较体部和底部表达降低。在纤维、脂肪、平滑肌、淋巴组织及脑组织中无明显表达。PIG11蛋白在肝癌、低分化胃和肠腺癌、乳腺癌组织中较相应正常组织均有明显表达下调(P<0.01);在鳞癌组织中,与正常鳞状上皮比较,也有表达下调(P<0.05)。结论PIG11蛋白主要分布在人体上皮组织,且与细胞的分化程度有关;PIG11蛋白低表达与上皮源性的恶性肿瘤发生发展有关。更多还原

【Abstract】 Objective To investigate the expression of PIG11(p53-induced gene 11)protein in human normal tissue and corresponding tumor tissue,discussing the distributing characteristic of PIG11 protein in human normal tissue and its function in the tumorigenesis and development.Methods The expression of PIG11 protein in 319 cases of human normal tissues and corresponding tumor tissues was determined by immunohistochemical peroxidase-conjugated streptavidin(SP) method.Results The expression of PIG11 protein was positive in human gastric mucosa,intestine mucosa,liver,galactophore glandular epithelium,and squamous epithelium tissue.Expression of PIG11 protein in cervical part of normal gastric mucosa gland was lower than that of caudomedial part and bottom.Its expression was negative in human fibre,fat tissue,smooth muscle,lymph tissue and brain tissue.Expression of PIG11 protein was descent in liver cancer,poorly differentiated stomach adenocarcinoma,poorly differentiated intestine adenocarcinoma,breast cancer,compared with those of corresponding normal tissues(P<0.01);and its expression was low in squamous carcinoma too,compared with normal squamous epithelium(P<0.05).Conclusion These results suggest that PIG11 protein is distributed in human normal epidermic tissues and expression of PIG11 protein is involved in cellular differentiation.Down-regulation of PIG11 protein is connected with tumorigenesis and development in epidermic malignant tumors.更多还原