【摘要】 目的建立大鼠腹主动脉移植慢性排斥反应的模型,观察移植动脉的变化规律。方法应用病理图像分析移植后动脉慢性排斥的变化,并结合免疫组化观察转化生长因子-1β(TGF-β1)在慢性排斥反应中的表达。结果移植术后动脉内膜增生并持续增厚,TGF-β1在移植动脉内膜大量表达并与病理改变相关。结论①大鼠移植动脉变化具有内膜增生,中层细胞坏死、迁移,炎性细胞浸润等特点。②TGF-β1在慢性排斥的发生发展中起着重要作用。更多还原

【Abstract】 Objective To establish the experimental animal model of transplantation rejection and observe the performance of transplanted of abdominal aorta tissues.Methods The rat model of artery allograft was deve-loped.The grafts were collected to receive immunohistochemical and histopathological examinations and quantitative measurement of TGF-β₁ expression.Results The thickening appeared after operation,strongly expressed TGF-β₁ in intima of artery during the episode of rejection.Conclusion Three characteristics including intima hyperplasia,media necrosis and cell migration and inflammatory cell infiltration are demonstrated in the model.The expression level of TGF-β₁ was related to the occurrence and development of rejection.更多还原