【摘要】 采用毛细管电泳高频电导法测定了未衍生化的卡托普利。考察了分离检测条件的影响。实验选择10.00mmol/L Tris-5.0mmol/LH3BO3-20.0%CH3OH为电泳介质,在优化条件下,卡托普利的线性范围为1.00μg/mL~200μg/mL,检出限为0.3μg/mL。成功地检测了血清和尿液中的卡托普利。更多还原

【Abstract】 Determination of underivatized captopril by capillary electrophoresis with high frequency conductivity detection was described.The key factors for separation and detection were studied and the best analysis conditions were obtained.In the buffer of 10.0mmol/L Tris-5.0mmol/L boric acid—20.0%CH₃OH,captopril in the samples could be separated and detected successfully.Under the chosen conditions,there was a linear relationship between the captopril concentration and the peak area in the range of 1.00μg/mL—200μg/mL.The limit of detection was 0.3μg/mL.The method was used for analysis of captopril in human serum and urine samples satisfactorily with the recovery of 98.7—103%.更多还原