【摘要】 目的观察银杏提取液对嗅神经鞘细胞(OECs)中的酪氨酸蛋白激酶(PTK)活性的影响。方法鼠龄14d的SD大鼠,断头处死,无菌条件下取出嗅球,夹碎嗅球,剔除白质部分,用虹膜刀剪碎后,原代培养。原代培养的OECs,贴壁2d后,将组织块软瓶培养2~3次,分别用按11600、13200、16400、112800稀释的银杏提取液37℃继续培养。以神经生长因子作为阳性对照;以DMEM作阴性对照。分别于培养后第24h、48h、72h和96h收集细胞,进行形态学观察;使用[ly-32P]ATP掺入,液闪计数法测定PTK活性。结果阴性对照组OECs的PTK无活性,阳性对照组PTK活性升高。银杏提取液组第48hPTK活性升高(P<0.01),随后逐渐降低,至96hPTK无活性。结论银杏提取液有促进OECs分化的功能。更多还原

【Abstract】 Aim: To explore the effect of the extract of Ginkgo biloba on olfactory enshenthing cell (OECs) differentiation in vitro. Methods:OECs were obtained from olfactory bulb of SD rats and cultured with the extract of Ginkgo biloba at dilution concentrations of 1GA6FA1 600, 1GA6FA3 200, 1GA6FA6 400, 1GA6FA12 800. Nerve growth factor was used as positive control and DMEM as negative one. After 24 h,48 h,72 h and 96 h, the OECs been captured, and the activity of protein tyrosine kinase(PTK) was examined by lyGAAB2 32 P ATP in corporation test using PTK assay system.Results: OECs in the negtive control group had no PTK activity,but that of positive control group was detected notably. The PTK activity in extract of Ginkgo biloba groups increased at 48 h (P<0.01) and decreased gradually. Conclusion: Extracts of the Ginkgo biloba can promote the differentiation of OECs.更多还原