【摘要】 目的探讨人脐血来源的CD34+干细胞治疗脊髓损伤的可行性,为脊髓功能障碍性疾病的临床治疗打下基础。方法用密度梯度离心及免疫磁珠法分离出人CD34+脐血干细胞,在含有B27、碱性成纤维细胞生长因子(bFGF)、人重组干细胞因子(rhSCF)的营养液中培养,用核荧光染色剂Hoechst33258标记细胞;制作大鼠脊髓横断模型,造成大鼠下肢瘫痪,在脊髓损伤3d移植荧光标记的CD34+脐血干细胞,观察移植后的动物行为变化、脊髓组织形态学变化等。结果脊髓损伤大鼠移植CD34+脐血干细胞5~6d可见瘫痪大鼠的后肢肌力开始恢复,10~15d后可出现爬行,3~4周后后肢活动活跃;对照组瘫痪的肢体未见恢复。脊髓移植区肉眼可见有增生的组织充填,镜下有大量神经胶质原纤维酸性蛋白、神经元特异性烯醇化酶阳性的细胞,移植区Hoechst33258荧光标记阳性细胞增生。结论移植的CD34+脐血干细胞可在移植部位增殖,并使脊髓横断大鼠运动功能恢复,有望成为治疗脊髓损伤的有效手段。更多还原

【Abstract】 Aim: To explore the effects of human umbilical cord blood stem cells on neurological recovery after spinal cord injury. Methods: CD34+ cells were isolated from human umbilical cord blood(HUCB) through a MACS magnetic separation column and cultured in a serum free medium(DMEM/F12) containing B27, bFGF and rhSCF.The cells were labeled by Hoechst33258.The spinal cord injury model was built by complete transecting rat spinal cord.The transplantation of cultured CD34+ cells were performed on the 3rd d after injury.The effect was evaluated with histological examination and restoration of function in the lesion area.Results: Four weeks after the transplantation,a large number of GFAP-positive cells and NSE-positive cells occupied the lesion,some of cells differentiated and extended into the surrounding spinal cord tissue.Histological evaluation revealed that Hoechst33258-labeled CD34~+ cells survived and migrated into the injured area.These rats restored the function of movement. Conclusion: The CD34+ HUCB stem cells transplantation provides a useful repair strategy for the mammalians with spinal cord injury and will bring a new therapy means for the clinical patients.更多还原