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层析法分离提取葡萄籽原花青素的进一步研究
Study on the Adsorption Behavior of Proanthocyanidin Extracted from Grape Seed on Macroporous Adsorbent Resin
【摘要】 以吸附量和解吸率为指标,比较了7种大孔吸附树脂对原花青素的静态吸附解吸性能并考查了AB—8树脂对原花青素的吸附特性及机理。实验结果表明:AB—8树脂是较理想的吸附剂;在溶液中AB—8树脂对原花青素的吸附达到平衡的时间为240 min,吸附行为符合Langmuir等温方程;最佳动态吸附参数原花青素提取液浓度4.0 mg/mL,吸附流速2 BV/h(1BV=13.03 mL)用40%乙醇溶液洗脱。AB—8树脂适合于柱层析操作分离原花青素。
【Abstract】 In this thesis,the adsorption and desorption performance of procyanidins on 7 kinds of macroporous adsorbent resins were compared and the adsorption behavior and mechanism of AB—8 resin were studied by the measure of adsorption capacity and desorption ratio.Experiments results indicated as follows:AB—8 resin was an excellent adsorbent resin.Under experiment conditions,the adsorption equilibrium time was 240 min.The results indicated that adsorption isotherm was correlated with Langmuir-type equation.The optimum conditions of dynamic adsorption were that concentration of procyanidins was 4.0 mg/mL,adsorption velocity was 2.0 BV/h,and desorption agent was 40% ethanol solution.AB—8 resin was excellent for isolation of procyanidins from grapeseeds.
【Key words】 grape seeds procyanidins ultrasonic wave macroporous adsorbent resin;
- 【文献出处】 化工时刊 ,Chemical Industry Times , 编辑部邮箱 ,2006年06期
- 【分类号】TQ28
- 【被引频次】15
- 【下载频次】490