【摘要】 小麦嘌呤吲哚蛋白(Puroindoline)是优质遗传特性硬度的生化标记,对小麦磨粉品质起决定性作用。对目前分析此类蛋白的两类Triton-X-114和Tricine-SDS-PAGE系统进行了优化和比较,建立了详细的适合半子粒分离Pin蛋白的电泳方法。采用此法检测转pinA基因的硬粒小麦P34的后代以及转基因硬粒小麦之间杂交子代的外源基因pinA的表达情况,取得了良好的效果。该方法为Puroindoline蛋白的进一步研究奠定了基础,为小麦品质生化和分子标记辅助育种提供了方法。更多还原

【Abstract】 Puroindoline protein is a biochemical marker for grain hardness and plays critical role in determining the milling property of wheat.Two efficient SDS-PAGE methods for analysing puroindoline protein including Triton-X-114 and Tricine systems were optimized,and were used to analyse the transgenic wheat line P34 containing transgene pinA and the F1 semi-single seeds of crossing.The results indicated that the improved method could study the puroindoline protein and screen the progenies of crossing efficiently which could be beneficial to wheat breeding program.更多还原