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转双抗虫基因741杨嫩枝扦插苗抗寒性的研究

Studies on cold resistance for softwood cutting seedling of transgenic hybird poplar 741 carrying two insect-resistant genes

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【作者】 李坤霞朱淑新张世红左永忠周怀军

【Author】 LI Kun-xia,ZHU Shu-xin,ZHANG Shi-hong,ZUO Yong-zhong,ZHOU Huai-jun (Agricultural University of Hebei,Baoding 071000,China)

【机构】 河北农业大学河北农业大学 河北保定071000河北保定071000

【摘要】 为研究转双抗虫基因741杨不同时期扦插苗的抗寒性,以5月、6月、7月、8月嫩枝扦插苗为试验材料,进行低温处理,测定生理反应和恢复生长能力。结果表明,7月、8月嫩枝扦插苗可溶性蛋白质含量低,-15℃以下低温相对电导率明显增高,枝条皮部逐渐变黑,芽失去萌发能力;而5月、6月嫩枝扦插苗对寒冷适应性较强,-15℃以下低温仍具一定恢复生长能力。

【Abstract】 To study cold resistance of softwood cutting of transgenic hybrid poplar 741 carrying two insect-resistant genes,softwood cutting seedlings in May,June,July and August were taken as experimental malterials for temperature treatment to measure physical reaction and comeback-growth ability.The result showed that the contents of water-solude protein in July and August were low,relative conductivity below-15℃ was obviously higher,the shoot became black gradually,the bud lost germination ability.However,softwood cutting seedlings in May and June were in better adapting to cold,and still had comeback-growth ability below-15℃.

【基金】 科技部农业转化基因项目(02EFN211300045);河北省科技攻关项目(01220135D)
  • 【文献出处】 河北林果研究 ,Hebei Journal of Forestry and Orchard Research , 编辑部邮箱 ,2006年03期
  • 【分类号】S792.11
  • 【被引频次】1
  • 【下载频次】51
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