【摘要】 为了研究银杏内生真菌的刺盘孢菌株的遗传分化,选用了分离自江苏5个县市的14个菌株,利用RAPD技术进行遗传多样性分析。从20个随机引物中筛选出5个引物,扩增出62条DNA带,在此基础上分析并建构了遗传相关聚类图。结果表明,不同地区的刺盘孢菌株其遗传差异性不同,14个菌株可聚为两大类,其中启东、邳州的6株聚为一类,南京、南通和泰兴的8株又聚为一类。所有的14个菌株的相似性系数范围在46%~89%。更多还原

【Abstract】 Random amplified polymorphic DNA(RAPD) was used to analyze genetic variability offourteen strains of Colletotrichum sp.,which were isolated from Ginkgo biloba L.in Jangsu provinceof China.62 bands of RAPD products were detected by means of 5 primers screened from 20 testedprimers.By UPGMA illustration,the results showed that 4 isolates from Qidong and 2 isolates from Pizhou had a higher genetic relativity with more than 70% similarity indexes among them,and 6 isolates from Nanjing,1 isolate from Nantong and 1 isolate from Taixing had a higher genetic relativity.Genetic similarity indexes of all strains varied from 46% to 89%.更多还原