【摘要】 目的：探讨阿霉素诱导的心肌病大鼠肾脏及心肌组织中血管紧张素转换酶2(ACE2)mRNA、血管紧张素转换酶(ACE)mRNA的表达及缬沙坦对其的影响。方法：30只Wistar大鼠随机分为3组：正常对照组即生理盐水组(n=10)；生理盐水+阿霉素组(n=10)；阿霉素+缬沙坦组(n=10)。RT-PCR法检测肾、心肌组织中ACE2 mRNA、ACE mRNA的表达,光镜及透射电镜观察心肌病理变化。结果：阿霉素组大鼠较正常对照组大鼠肾脏及心肌组织中ACE2 mRNA(3．35±0．39对2．21±0．18,P=0．000；2．50±0．29对1．25±0．13,P=0．000)、ACE mRNA (2．31±0．38对1．19±0．12,P=0．000；1．77±0．09对0．84±0．11,P=0．000)的表达均明显增高,缬沙坦治疗组大鼠肾脏及心肌组织中ACE2 mRNA(2．65±0．34对3．35±0．39,P=0．001；1．72±0．32对2．50±0．29,P=0．000)、ACE mRNA (1．68±0．15对2．31±0．38,P=0．004；1．21±0．10对1．77±0．09,P=0．000)的表达较阿霉素组明显降低。结论：阿霉素诱导的心肌病大鼠肾脏及心肌组织中ACE2 mRNA、ACE mRNA表达水平均显著高于正常大鼠；缬沙坦对于阿霉素诱导的心肌病大鼠肾脏及心肌具有保护作用。更多还原

【Abstract】 Objective:To determine the levels of mRNA expression of ACE2 and ACE on doxorubicin（DOX）-induced cardiomyopathy in rats,and to explore its changes with valsartan. Methods:Twenty adult Wistar rats were given DOX(2.5 mg·kg^-1 i.p.twice weekly).Four weeks after the administration of DOX,10 rats were treated with valsartan(400 mg·kg^-1,p.o.daily) for 6 weeks,and 10 rats received saline were used as a control group.The levels of mRNA expression of ACE2 and ACE were examined by RT-PCR.Histology was performed in DOX-rats after I0 weeks in both groups.Results:ACE2 mRNA（kidney,3.35±0.39 vs 2.21±0.18,P=0.000;heart,2.50±0.29 vs 1.25±0.13,P=0.000） and ACE mRNA（kidney,2.31±0.38 vs 1.19±0.12,P:0.000; heart,1.77±0.09 vs 0.84±0.11,P=0.000） was significantly increased in the DOX-induced kidney and heart as compared to that in the controls.The expression of ACE2 and ACE mRNA in DOX-rats was decseased with valsartan treatment（ACE2:kidney,2.65±0.34 vs 3.35±0.39,P=0.001; heart,1.72±0.32 vs 2.50±0.29,P=0.000;ACE:kidney,1.68±0.15 vs 2.31±0.38,P= 0.004;heart,1.21±0.10 vs 1.77±0.09,P=0.000）.Conclusions:These data indicate that ACE2 is up-regulated in DOX-induced cardiomyopathy.Valsartan could protect the kidney and heart during cardiomyopathy of rat.更多还原