【摘要】 采用高频密码子分析法,对柑橘的177个蛋白质编码基因序列(codingDNAsequence,CDS)进行了分析,计算出同义密码子相对使用频率(relativefrequencyofsynonymouscodon,RFSC),确定了TAA、GCT、GAT、CTT、AGG、AGA和GTT等7个高频密码子。将柑橘的密码子使用频率与人、果蝇、酵母和大肠杆菌等不同种类模式生物比较后发现,柑橘密码子的偏爱性与不同种类生物有不同程度的差异;但将柑橘的密码子使用频率与拟南芥、番茄、水稻和尖叶蕉等不同种类的植物相比,发现柑橘密码子的偏爱性与同为双子叶植物的拟南芥、番茄完全一样,而与水稻、尖叶蕉这2种单子叶植物均有较大的差异。分析结果对动物或微生物的基因在柑橘中的表达或柑橘基因在微生物中的表达以及基因克隆时设计引物具有一定指导意义。更多还原

【Abstract】 177 coding DNA sequences (CDS) were used to analyze the relative frequency of synonymous codon (RFSC), and 7 high-frequency codons including TAA, GCT, GAT, CTT, AGG, AGA and GTT were revealed by high-frequency codon analysis. When the frequency of codon usage of citrus was compared to Homo sapiens, Drosophila melanogaster, Saccharomyces cerevisiae and Escherichia coli, we found that the codon preference is considerably different between citrus and non-plant species. While compared to Arabidopsis thaliana, Lycopersicon esculentum, Oryza sativa and Musa acuminata, we found that the codon preference was identical in dicotyledons, but was different between citrus and monocotyledon, Oryza sativa and Musa acuminate. The data are helpful to researches involving introducing a non-plant gene to citrus or vice versa and designing primers for functional gene cloning.更多还原