【摘要】 目的探讨中华眼镜蛇毒C组分(FC)对胶质瘤细胞株(U-251细胞株)的抑制作用。方法应用MTT法,观察NajaNajaAtraVenom(NNAV)FC对人胶质细胞瘤株U-251的细胞毒作用、量效关系等,并与vm-26对上述细胞的细胞毒作用进行比较。结果FC对人胶质瘤细胞有明显的抑制作用,其24h及48h的IC50分别为5.76μg/ml和7.19μg/ml,且呈良好的量效关系。结论FC能有效抑制胶质瘤细胞株的生长,抑制作用与浓度呈正相关,与卫萌相比较,FC是有效的、能抑制胶质瘤细胞生长的药物。更多还原

【Abstract】 ObjectiveTo investigate the inhibition of fraction C (FC) from Naja Naja Atra Venom (NNAV) on U-251 glioma cell line. MethodsMTT was involved in detecting the cytotoxic effects, dose-effect related rates of FC on human glioma cell line (U-251), These effects were compared with VM-26 on the above mentioned cells. ResultsThe proliferation of U-251 was obviously inhibited by FC in a dose dependent manner. The inhibitive effect of FC on U-251 was obvious, with the IC50 5.76 ug/ml～7.19 ug/ml at 24 h and 48 h respectively. ConclusionFC can inhibit the proliferation of U-251.更多还原