【摘要】 探讨白黎芦醇(Res)对人晶状体上皮细胞(HLEC)增殖的影响。体外培养HLEC 24h,加入重组人碱性成纤维细胞生长因子(终浓度20μg/L)使HLEC处于增殖状态。MTT比色法检测不同浓度Res作用于增殖状态的HLEC 24h及25mg/L Res作用于增殖状态HLEC 6h、12h、24h、48h对细胞增殖的影响。结果表明不同浓度的Res,25mg/L Res于不同时间对增殖状态的HLEC有明显的抑制作用,并具有时间及剂量依赖关系。提示Res能够明显抑制HLEC增殖,有望成为防治后发性白内障的有效药物。更多还原

【Abstract】 To study the effect of resveratrol on proliferation of human lens epithelia cells(HLEC).HLEC were incubated in vitro for 24 hours,added to recombinant human basic fibroblast growth factor(rhbFGF)(final concentration 20μg/L),and then HLEC were in proliferation.Different concentrations of resveratrol were added to the proliferative HLEC separately for 24 hours,and 25mg/L resveratrols were added to the proliferative HLEC separately for 6h,12h,24h,and 48h.The effect of proliferation of resveratrol on the HLEC was determined by methyl thiazolyl tetrazolium(MTT) colorimetric assay.The results showed that different concentrations of resveratrol,25mg/L resveratrol in different time can significantly inhibit the proliferation of HLEC,and these effects were increased in both time-dependent and dose-dependent ways.These indicate that resveratrol could effectively inhibit the proliferation of HLEC in vitro,and it might become reliable and effective drug to prevent and cure aftercataract.更多还原