【摘要】 用荧光光谱法研究了卡马西平与牛血清白蛋白的结合反应的热力学特性,并发现卡马西平对牛血清白蛋白有较强的荧光猝灭作用。从荧光猝灭光谱数据,由stern-volmer方程、double-reciprocal方程和热力学公式,得到了25℃时结合反应的结合常数为2.047×104mol.L-1,其△Hθ、△Gθ和△Sθ分别为-14.46 kJ.mol-1-、32.88kJ.mol-1和-24.03 J.K-1。更多还原

【Abstract】 The thermodynamic characteristic for binding reaction of carbamazepine to bovine serum albumin（BSA） was studied using fluorescence spectroscopy.It was shown that carbamazepine has a powerful ability to quench the BSA fluorescence via a nonradiative energy transfer mechanism.The fluorescence quenching data was analyzed according to Stern-Volmer equation and double-reciprocal equation.At 25℃,the binding constant was 2.047×10⁴mol·L^-1,and the thermo-dynamic parameters △H^θ,△G^θ and △S^θ were-14.46 kJ·mol^-1,-32.88 kJ·mol^-1 and-24.03 J·K^-1,respeetively.更多还原