【摘要】 用循环伏安法制备了聚L-赖氨酸修饰玻碳电极,研究多巴胺在聚L-赖氨酸修饰电极上的电化学行为,建立了循环伏安法测定多巴胺的新方法。实验结果表明,在pH7.0的磷酸盐缓冲溶液中,扫描速率为150mV/s,循环扫描电位在-0.3~0.6V时,多巴胺在聚L-赖氨酸修饰玻碳电极上出现一对灵敏的氧化还原峰,峰电位分别为Epa=0.175V,Epc=0.146V(相对饱和甘汞电极);测定多巴胺的线性范围为1.0×10-3~1.0×10-5mol/L和1.0×10-5~8.0×10-9mol/L,方法检出限1.0×10-9mol/L。用于药剂中多巴胺的测定。更多还原

【Abstract】 The poly (L-lysine) modified electrode was prepared by using cyclic voltammetric method. The voltammetric behavior of dopamine and cyclic voltammetric method for the determination of trace dopamine have been studied on the poly (L-lysine) modified electrode. In pH 7.0 phosphate buffer solution,the peak potentials were 0.175 V of E_ pa ,and 0.146 V of E_ pc (vs.SCE) at the scan rate 150 mV/s and cyclic scan potential range from -300 mV to 600 mV. The linear ranges of the method were 1.0×10 -3 -1.0×10 -5 mol/L and 1.0×10 -5 -8.0×10 -9 mol/L. The detection limit was 1.0×10 -9 mol/L. The method was applied to the determination of dopamine in the drug with satisfactory results.更多还原