【摘要】 在胶束电动色谱模式下,考察了影响核苷分离的缓冲溶液、SDS浓度、pH、表面活性剂、有机添加剂、分离温度及外加电压等重要因素,并优化了关键的分离条件,建立了一种简单快速的利用毛细管胶束电动色谱DAD检测器分离分析核苷的新方法。当缓冲溶液为36 mmol/L硼酸缓冲液(pH 9.0)、30 mmol/L SDS和3%(V/V)乙腈,分离电压为25 kV和分离温度为25℃时,5种核苷在6 m in内实现了令人满意的基线分离。在优化的条件下,对其线性范围、检出限和重现性进行了测定。结果表明,核苷的迁移时间的重现性<1%;面积的重现性<3%。所建立的分离方法的线性范围宽,检出限低,重现性好。更多还原

【Abstract】 A novel method for the separation of nucleotides by micellar electrokinetic chromatography with(diode) array detection was developed.The effect of some key factors,such as concentration of buffer and(sodium) dodecyl sulfate(SDS),pH,surfactant,addition of organic solvent,capillary temperature and applied voltage on the separation of nucleotides was investigated.Under the optimized conditions,the baseline separation of five nucleotides was achieved with satisfactory repeatability and high separation efficiency in 6 min(using) 36 mmol/L borate buffer(pH 9.0) containing 3%(V/V) acetonitrile and 30 mmol/LSDS,under(25 kV) applied voltage and at 25℃.The linear range,the limit of detection and the repeatability were investigated under the optimized conditions.The RSDs(n=6) of the migration time and peak area for each nucleotide are less than 1% and 3%,respectively.更多还原