【摘要】 以玻碳电极为基体,制备了单壁碳纳米管复合聚吖啶橙(SWNTs/POAO)修饰电极。此电极结合纳米材料电催化特性和吖啶橙聚合物薄膜的分子识别能力,以脱氧核糖核酸(DNA)分子中的碱基对为探针,研究了DNA中碱基对的电化学行为。该电极对腺嘌呤(ADE)、鸟嘌呤(GUA)、胞嘧啶(CYT)和胸腺嘧啶(THY)都表现了良好的电催化性,嘌呤碱和嘧啶碱的氧化峰能够完全分离。在pH 7.0的B.R.缓冲溶液中,在0.5~1.3V电位范围内,GUA和ADE分别在0.7V和1.0 V左右出现灵敏的氧化峰,峰电流随其浓度而线性变化。ADE和GUA的检出限分别为1.8×10-9和9.0×10-10mol/L。嘧啶碱基不干扰嘌呤碱基的测定。SWNTs/POAO电极可以用来同时测定DNA中的ADE和GUA。更多还原

【Abstract】 A novel single wall nanotube（SWNT） compound poly（acridine orange） modified electrode（SWNTs/POAOE） was prepared at glassy carbon electrode（GCE）,which combined the electrocatalytical（capability） of nanomaterials with the molecular recognition ability of polymer film,and it could be used for（simultaneous） determination of bases in deoxyribonucleic acid（DNA）.The SWNTs/POAOE showed excellent electrocatalytic activity toward electro-oxidation of adenine（ADE）,guanine（GUA）,cytosine（CYT） and（thymine）（THY）.The oxidation peaks of purines and pyrimidines could be entirely separated at a same SWNTs/POAOE,two well-defined oxidation peaks were observed at about 0.7 V for GUA and 1.0 V for ADE in pH 7.0 Bristton-Robinson（B.R.） buffer.The detection limits were 1.8×10^-9 mol/L for ADE,and 9.0×10^-10 mol/L for GUA. The pyrimidine bases did not interfere with the determination of ADE and GUA.This（electrode） presented here could be used for the simultaneous determination of ADE and GUA in DNA.更多还原