【摘要】 采用玻璃化法对不同基因型的龙眼胚性愈伤组织超低温保存进行了初步探讨.将保存一定时间的胚性愈伤组织于常温下用60%玻璃化溶液(2PVS2)装载30 m in,再于0℃下用PVS2溶液平衡60 m in;换新鲜的PVS2溶液,迅速投入液氮中保存;48 h后化冻.结果表明,具有原胚分化的胚性愈伤组织保存后的存活率明显高于普通胚性愈伤组织;40℃温水浴、25℃左右室温和自来水冲洗等3种不同的化冻方式对龙眼胚性愈伤组织玻璃化超低温保存后的存活率具有同样的效果.更多还原

【Abstract】 The procedure for cryopreservation by vitrification was preliminarily developed in different genotypes of longan calli.The calli were loaded with 60% 2PVS₂ for 30 minutes at room temperature,and then they were exposed to PVS₂ for 60 minutes at the temperature below 0 ℃.Soon afterwards,the calli were put into liquid nitrogen with the new solution of fresh PVS₂,and were thawed 48 hours later.In the experiment,three thawing methods,such as washing by water at 40 ℃,exposing at the room temperature of 25 ℃,and washing by tap water were used to estimate the effects of thawing methods on the survival rate after cryopreservation.The results showed that the relative survival rate of the calli with proembryos was higher than that of the general calli;and that different thawing methods had the same effect on the survival rate of longan calli by cryopreservation.更多还原